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Biomol GmbH ac-devd-afc fluorogenic substrate
Ac Devd Afc Fluorogenic Substrate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorogenic+substrate+ac-devd-afc/fluorogenic+substrate+ac+devd+amc/pmc03546013-207-7-10
Average 90 stars, based on 1 article reviews
ac-devd-afc fluorogenic substrate - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Incubation:

Article Title: Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues
Article Snippet: .. In brief, extracts were incubated with 10 μM either mammalian or yeast cytochrome c and 1 mM dATP at 37°C for 30 min before addition of the fluorogenic substrate, Ac-DEVD-afc (Biomol). .. Alternatively, extracts alone or with 8 μM cytochrome c were incubated as above before addition of the colorimetric substrate Ac-DEVD-pNA (Biomol).

Article Title: PTD-XIAP protects against cerebral ischemia by anti-apoptotic and transcriptional regulatory mechanisms.
Article Snippet: Caspases play a major role in the infarction process that follows occlusion of cerebral arteries and are important targets for stroke therapy.. We have generated three fusion proteins that link various domains of the X chromosome-linked inhibitor of apoptosis (XIAP), a potent caspase inhibitor, to the protein transduction domain (PTD) of HIV-1/Tat, and have tested their efficacy after distal occlusion of the middle cerebral artery (dMCAO) in mice.. PTD–XIAP failed to accumulate in brain structures after intravenous (iv) delivery, but properly transduced cortical cells when applied topically.

other:

Article Title: Defined inflammatory states in astrocyte cultures: correlation with susceptibility towards CD95-driven apoptosis.
Article Snippet: Caspase-3 activity was measured as the ability to cleave the fluorogenic substrate Ac-AspGlu-Val-Asp-7-amino-4-trifluoromethyl coumarin (Ac-DEVD-afc) (Biomol Research Laboratories, Plymouth Meeting, PA, USA).

Article Title: Estrogens Attenuate Oxidative Stress and the Differentiation and Apoptosis of Osteoblasts by DNA-Binding-Independent Actions of the ERα
Article Snippet: Apoptosis in cultured cells was determined by measuring caspase-3 activity by cleavage of the fluorogenic substrate Ac-DEVD-AFC (Biomol, Plymouth Meeting, PA, USA), as described previously. ( )

Article Title: Mechanical stimulation prevents osteocyte apoptosis: requirement of integrins, Src kinases, and ERKs.
Article Snippet: Caspase 3 activity was determined by measuring the degradation of the fluorogenic substrate Ac-DEVD-AFC (Biomol) in lysates of cells stretched for 10 min at 5% elongation followed by addition of etoposide for 9 h, as previously reported (53).

Caspase Assay:

Article Title: PTD-XIAP protects against cerebral ischemia by anti-apoptotic and transcriptional regulatory mechanisms.
Article Snippet: Caspases play a major role in the infarction process that follows occlusion of cerebral arteries and are important targets for stroke therapy.. We have generated three fusion proteins that link various domains of the X chromosome-linked inhibitor of apoptosis (XIAP), a potent caspase inhibitor, to the protein transduction domain (PTD) of HIV-1/Tat, and have tested their efficacy after distal occlusion of the middle cerebral artery (dMCAO) in mice.. PTD–XIAP failed to accumulate in brain structures after intravenous (iv) delivery, but properly transduced cortical cells when applied topically.



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